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Study on the Extraction of the Ovine Pancreatin Process and the Kinetics of Digestion of Casein

Posted by Coleman
TAGS: Ovine_PancreasPancreatinIsopropyl_alcohol
ExtractPrecipitateKinetics
Now production of pancreatin is mainly pig pancreatin,Ovine pancreatin in domestic blank, Ovine pancreatin is Muslim product for the Muslim consumers,The raw materials of the experiment is ovine pancreas, pancreatin extracting was studied by the methods of organic solvents, isopropyl alcohol as optimum extraction agent and precipitation agent.Through the single factor and the orthogonal test, the major effect factor and the process parameter of the pancreatin extracting method were studied, the main technical process and the parameter of pancreatin’s product has determined; protectant,activator and degreaser were studied;Through the pancreatin activities and physics chemistry Indicators were determined by the chinese pharmacopoeia,Kinetics of Ovine pancreatin hydrolyse casein were studied, the research results are as follows:1. Technical process: During the experiment which product ovine pancreatin by organic solvents method, take the frozen ovine pancreas as the raw material, during the pancreas broken,activation,extract,precipitation,remove fat,vacuum drying to get the pancreatin.2.Parameters of Extracting process: Optimizing activating time, activating temperature, activating pH value, extraction agent concentration, extracting temperature and extracting time of the best condition and parameters. Through the single factor test, determined the main impact factors and parameters, then using L18(37)orthogonal test, take the activities of trypsin as the index, screened out the best process parameter were: extraction agent concentration 10%,extracting temperature 15℃, extracting time 5h, activating pH6,activating temperature15℃and activating time 5h.3. Optimizing protectants and activators :Starch, maltose and sodium chloride as protectants and select the CaCl2 -Pancreatin for the best activator.4.Parameters of precipitating process : Optimizing Precipitation agent concentration, Precipitation temperature, Precipitation pH value and Static placed time of the best condition and parameters. Through the single factor test,Determined the main impact factors and parameters, then using L9(34)orthogonal test, take the activities of trypsin as the index, screened out the best process parameter were: Precipitation agent concentration 60%,Precipitation temperature 5℃,Precipitation pH value 6 , Static placed time1h.5. Optimizing degreaser : Acetone as the best degreaser . According to the confirmation test of above process parameter, the result indicated that the yield of pancreatin was 11.2%,the activities of trypsin,amylopsin,lipase respective were 2654 U/g,13800U/g,10400 U/g,Loss on drying 3.2%,Fat content 17.1mg/g, the results fit for the chinese pharmacopoeia.6. Kinetics of Ovine pancreatin hydrolyse casein were studied: The optimum temperature was measured 40℃, the optimum pH was measured 8.Through the research of Thermal stability, pancreatin activity decreased with the time gradually increased, The higher the temperature, the faster inactivation of trypsin, The poor thermal stability;Through the research of pH stability, pancreatin activity decreased with the time gradually increased, At the optimum pH, the fastest inactivation of trypsin, The poor pH stability. According to research the affect of trypsin activity by Ca2+,Na+and EDTA: Ca2+ can activate ovine pancreatin,and to improve the stability of the ovine pancreatin,Na+ does not affect the activity of trypsin, but EDTA has Strong Inhibition effect to the activity of trypsin.In summary,Using the isopropyl alcohol extraction ovine pancreatin powder,Simple process,higher yield ,higher activity of three enzymes, Lower cost and Short production cycle. Improve the production efficiency and the utilization rate of raw materials, conservation of resources, reduce pollution.Use advanced bioseparation engineering to produce pancreatin, increase the yield and enzyme activity which has positive significance in biochemical pharmaceutical industry and comprehensive utilization of animal by-products. Key words: Ovine Pancreas; Pancreatin; Isopropyl alcohol; Extract;
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